Journal: Endocrinology and Metabolism

Article Title: Effects of Oxytocin on Cell Proliferation in a Corticotroph Adenoma Cell Line

doi: 10.3803/EnM.2019.34.3.302

Figure Lengend Snippet: Expression of oxytocin (OXT) receptor in normal mouse pituitary gland and immunofluorescence staining of OXT receptor in AtT20 and GH3 cells. (A, B) Immunohistochemical staining shows that normal mouse pituitary gland was stained positively for OXT receptor ( n =2, ×40 and ×200, respectively). (C) It indicates positive control for OXT receptor staining in uterine endometrial tissue ( n =3, ×200). (D) It indicates immunofluorescence co-staining of OXT receptor (red color) and corticotropin-releasing hormone receptor (green color) in AtT20 cells (magnification, ×630). (E, F) These panels show the staining for OXT receptor (green color) in AtT20 and GH3 cells (×400), respectively. AL, anterior lobe.

Article Snippet: The effects of OXT (Cat No. 50-56-6, Cayman Chemical, Ann Arbor, MI, USA) or OXT receptor antagonist (L-368,899, Cat No. 1910, TOCRIS Bioscience, Bristol, UK) on proliferation of AtT20 and GH3 cells were determined by MTT assay.

Techniques: Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Positive Control

Journal: Endocrinology and Metabolism

Article Title: Effects of Oxytocin on Cell Proliferation in a Corticotroph Adenoma Cell Line

doi: 10.3803/EnM.2019.34.3.302

Figure Lengend Snippet: Cell cycle analysis in AtT20 cells. AtT20 cells were treated with oxytocin (OXT) and/or OXT receptor antagonist L-368,899 for 6 or 24 hours, and cells were stained with propidium iodide. DNA contents were analyzed by flow cytometer to assess cell cycle phases ( n =3). (A) Flow cytometry of AtT20 cells treated with OXT and/or OXT receptor antagonist for 6 or 24 hours. (B, C) Representative histogram data of the cell cycle analysis of AtT20 cells treated with OXT and/or OXT receptor antagonist for 6 or 24 hours. PI-A, Propidium iodide-Area. a P <0.05 vs. control group; b P <0.05 vs. L-368,899 treatment group.

Article Snippet: The effects of OXT (Cat No. 50-56-6, Cayman Chemical, Ann Arbor, MI, USA) or OXT receptor antagonist (L-368,899, Cat No. 1910, TOCRIS Bioscience, Bristol, UK) on proliferation of AtT20 and GH3 cells were determined by MTT assay.

Techniques: Cell Cycle Assay, Staining, Flow Cytometry, Control

Journal: Endocrinology and Metabolism

Article Title: Effects of Oxytocin on Cell Proliferation in a Corticotroph Adenoma Cell Line

doi: 10.3803/EnM.2019.34.3.302

Figure Lengend Snippet: Effects of oxytocin (OXT) on gene expression of (A) OXT receptors and (B) pro-opiomelanocortin (POMC) in AtT20 cells. AtT20 cells were exposed to OXT (50 nM) for 30 minutes, 1, 3, 6, 12, and 24 hours. mRNA levels of POMC and OXT receptor genes were estimated at each time point using quantitative real-time polymerase chain reaction. Data are presented as mean±standard deviation ( n =4 to 6). a P <0.05 vs. control group.

Article Snippet: The effects of OXT (Cat No. 50-56-6, Cayman Chemical, Ann Arbor, MI, USA) or OXT receptor antagonist (L-368,899, Cat No. 1910, TOCRIS Bioscience, Bristol, UK) on proliferation of AtT20 and GH3 cells were determined by MTT assay.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Standard Deviation, Control

Journal: Endocrinology and Metabolism

Article Title: Effects of Oxytocin on Cell Proliferation in a Corticotroph Adenoma Cell Line

doi: 10.3803/EnM.2019.34.3.302

Figure Lengend Snippet: Effects of oxytocin (OXT) on proliferating cell nuclear antigen (PCNA) and extracellular-signal-regulated kinase 1/2 (ERK1/2) in AtT20 cells. AtT20 cells were incubated with (A) 50 nM OXT and/or (B) 10 nM OXT receptor antagonist L-368,899 for 24 hours, and protein levels of PCNA and ERK1/2 in cells were examined by Western blot analysis. Data are presented as mean±standard error of the mean ( n =3). P-ERK, phosphorylated extracellular-signal-regulated kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. a P <0.05 vs. control group.

Article Snippet: The effects of OXT (Cat No. 50-56-6, Cayman Chemical, Ann Arbor, MI, USA) or OXT receptor antagonist (L-368,899, Cat No. 1910, TOCRIS Bioscience, Bristol, UK) on proliferation of AtT20 and GH3 cells were determined by MTT assay.

Techniques: Incubation, Western Blot, Control

Journal: Endocrinology and Metabolism

Article Title: Effects of Oxytocin on Cell Proliferation in a Corticotroph Adenoma Cell Line

doi: 10.3803/EnM.2019.34.3.302

Figure Lengend Snippet: Effects of oxytocin (OXT) on proliferating cell nuclear antigen (PCNA) and extracellular-signal-regulated kinase 1/2 (ERK1/2) in GH3 cells. GH3 cells were incubated with (A) 50 nM OXT and/or (B) 10 nM OXT receptor antagonist L-368,899 for 24 hours, and protein levels of PCNA and ERK1/2 in cells were examined by Western blot analysis. Data are presented as mean±standard error of the mean ( n =3). P-ERK, phosphorylated extracellular-signal-regulated kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. a P <0.05 vs. control group.

Article Snippet: The effects of OXT (Cat No. 50-56-6, Cayman Chemical, Ann Arbor, MI, USA) or OXT receptor antagonist (L-368,899, Cat No. 1910, TOCRIS Bioscience, Bristol, UK) on proliferation of AtT20 and GH3 cells were determined by MTT assay.

Techniques: Incubation, Western Blot, Control